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Dako Autostainer Plus | User Guide 75

Compatibility Check

A compatibility check has been built into the Dako Autostainer Plus software to minimize programming

errors resulting from assigning incompatible reagents (such as alkaline phosphatase-labeled

streptavidin and DAB substrate) to a slide protocol. This feature has two check levels. The first check

is the species reactivity compatibility of the primary antibody and the secondary reagent used. The

second addresses the compatibility rules dictated by the enzyme used in the detection system. The

following tables summarize the compatibility rules and codes assigned to each reagent type.

Every reagent in the reagent categories lists (Protocol Element) can be programmed with an

appropriate compatibility code or combination of codes. For example a linking reagent that reacts with

mouse and rabbit can be marked with compatibility codes AB. The software verifies that these

compatibility rules are not violated during the programming of a staining run. If an incompatible

reagent is assigned to a tile in the programming grid, an INCOMPATIBLE REAGENTS dialog box

displays the two reagents which are incompatible.

Select the NO button to replace an incompatible reagent. The incompatible reagent is removed from

the last programmed tile and the reagent list corresponding to that tile is displayed.

Select the YES button to override the warning message. The next available tile for programming

flashes and the corresponding reagent list is displayed.

A. Species Reactivity

Reagent Type (Protocol

Element)

Description Compatibility

Code

Primary Antibody All monoclonal primary antibodies raised

in mouse (e/b., mouse anti-human

Primary Antibody All polyclonal primary antibodies raised in

rabbit (e.g., rabbit anti-human

Secondary Reagent All secondary reagents (antibodies)

compatible with monoclonal antibodies

raised in mouse (e.g., biotinylated anti-

mouse lgG)

Secondary Reagent All secondary reagents (antibodies

reacting with polyclonal antibodies raised

in rabbit (e.g., biotinylated anti-rabbit lgG)

Secondary Reagent All secondary reagents (antibodies)

reacting with moth monoclonal and

polyclonal antibodies raised in mouse or

rabbit, respectively.

B. Enzyme System Compatibility

Reagent Type (Protocol

Element)

Description Compatibility

Code

Endogenous Enzyme Block, HRP-compatible X

Endogenous Enzyme Block, AP-compatible Y

Tertiary Reagent HRP-labeled streptavidin X

Tertiary Reagent AP-labeled streptavidin Y

Substrate HRP-compatible substrates (i.e., DAB,

AEC)

Substrate AP-compatible substrates (i.e., Fast Red,

New Fuchsin)

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