Dako Autostainer Plus Manual do Utilizador descarregar pdf (Página 70)

70 Dako Autostainer Plus | User Guide

5. Enter the correct compatibility code and press TAB or ENTER. Assigning compatibility

information to reagents is optional (See Compatibility Check at the end of this section).

The cursor moves to the Lot box.

6. Enter the reagent lot number and press TAB or ENTER. The lot # cannot exceed 8 alpha-

numeric characters. Assigning lot number information to reagents is optional. The cursor

moves to the Exp. Date box.

7. Enter the reagent expiration date and press TAB or ENTER. The reagent expiration date

entry is optional. To enter, use XXYY where XX is a two-digit numeric character

symbolizing a month and YY signifies a year. A maximum of 5 characters can be entered.

The entry of expired reagents is not accepted by the software. The cursor moves to the

Time box.

NOTE: All expiration dates entered are limited to a maximum of 10 years from the date of

entry.

8. Enter the reagent incubation time and press TAB or ENTER. The cursor moves the

Reagent Name box to allow addition of the next reagent within the same reagent

category. Follow steps 3-8 until all reagents have been added.

NOTE: A minimum of 2 minutes incubation time is required for proper operating

conditions.

9. Select the OK button to add the new reagent(s) to the reagent list. The system also

prompts “Save changes on disk?”. Select the YES button and the new reagent is saved to

the appropriate list. Select the NO button and the new reagent is not saved. The

PROGRAMMING GRID is displayed.

Select the CANCEL button to escape without saving. The PROGRAMMING GRID is

displayed.

Adding Primary Antibodies

A primary antibody can be added to the reagent list with a specific pretreatment reagent. This will

facilitate automatic selection of the pretreatment whenever a primary antibody is selected at the

PROGRAMMING GRID.

1. From the PROGRAMMING GRID select Edit lists from the menu bar. The reagent lists

categories are displayed.

2. Select the Primary Antibody category. The Primary Antibody EDIT REAGENT LIST

screen is displayed with the cursor in the text box in the Antibody section.

NOTE: All symbol characters such as (*), (:), (/) and (-), etc. are incompatible with the

Autostainer Plus software. Do not use any symbol characters. The use of symbol

characters may interrupt the Autostainer Plus during operation.

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Dako Autostainer Plus Manual do Utilizador Página 70